Human test of hydrogen water to protect skin and anti-aging
South Korean scholars have used animal experiments to prove that "Hydrogen Bathing Can Fight Against Ultraviolet Rays" causes skin damage to animals. Scholars from Hiroshima University in Japan verified this hypothesis using cytology and human experiments, proving that hydrogen water bathing can alleviate the generation of skin wrinkles, suggesting that hydrogen water has the anti-aging effect of beauty and beauty.
Oxidative stress is the oxidative damage caused by the oxidation and destruction of biological molecules by free radicals. Clinical verification work has been carried out in various aspects such as dyslipidemia, Parkinson's, stroke, myocardial infarction, and advanced cancer.
However, in the history of electrolysis water research, Shirahata et al. began to believe that hydrogen molecules were too stable to produce antioxidant activity. The hydrogen atoms in electrolyzed water are active hydrogen that can truly exert antioxidant effects, and they have conducted a number of confirmatory studies. Later, although it was thought that hydrogen and hydrogen atoms worked together. However, the idea of hydrogen atom or active hydrogen has been gradually ignored as the hydrogen effect has been repeatedly proved, but no one has clearly denied or corrected this idea. This study also uses electrolyzed water, but the study uses hydrogen content as the standard for the effect of electrolyzed water, so it can be considered that scholars who study electrolyzed water have already assumed that the essential role of electrolyzed water is hydrogen, not active hydrogen atoms.
In this study, in vitro cytology was used and human experiments were conducted. Using warm electrolysis hydrogen-rich water, the temperature is 41 degrees, the dissolved amount of hydrogen is 1.13 ppm, and the oxidation-reduction potential ORP is -741mV. The content of hydrogen in ordinary water is below 0.01 ppm (not measured), and the oxidation-reduction potential is +184 mV. For skin fibroblast OUMS-36 and keratinocyte HaCaT for UV damage test,
This kind of experiment can be considered to simulate human skin being damaged by ultraviolet radiation. The results showed that hydrogen water increased the synthesis of type I collagen in OUMS-36 cells by 1.85 to 2.03 times after 3-5 days. Immunohistochemical staining showed that type I collagen was mainly distributed around the nucleus. Collagen is an important functional protein of the skin. It is synthesized by skin cells. Increased synthesis of collagen is often a stress response when the skin is damaged. The amount of synthesis is related to the degree of damage. This experiment is to show that the cells treated with UV rays are indeed damaged.
The results of cell proliferation and cytotoxicity (WST-1) and apoptosis and necrosis (Hoechst 33342) found that
Cell DNA damage and apoptosis and necrosis are also the consequences of UV damage, which can further indicate that the damage caused by UV is severe enough and very destructive, and some cells have been destroyed and died. The role of hydrogen is to prevent this damage from happening.
The photochemical reduction method of nitrotetrazolium blue chloride (NBT) proved that hydrogen has an inhibitory effect on the production of superoxide anions in both cells. This is a study showing the level of reactive oxygen species. Ultraviolet rays damage skin cells. The middle is the process of oxidative damage. The increase of reactive oxygen species indicates the existence of oxidative stress.
Hydrogen treatment can reduce this level of active oxygen, indicating that hydrogen has the effect of anti-oxidative damage.
However, in many hydrogen studies, the selective anti-oxidation of hydrogen is not taken too seriously. It should be noted that the active oxygen here is mainly superoxide anion. According to the logic of selecting anti-oxidation, hydrogen cannot be directly neutralized.
The above is a cytological study, the results suggest that hydrogen has the effect of anti-ultraviolet damage, and then a human test was carried out to verify this concept.
Test site and hydrogen concentration analysis
The human body anti-wrinkle effect test uses hydrogen water bathing. 6 Japanese people participated in the bathing with water with a hydrogen content of 0.2-0.4 ppm every day for 3 months. By comparing the neck and back skin on the 90th day with the test, it was found that the neck and back skin wrinkles of 4 subjects were significantly reduced. Research suggests that hydrogen water washing can be used as a daily skin protection against ultraviolet radiation damage and anti-wrinkle measures by removing active oxygen.
The skin is enlarged and the depth of wrinkles is compared. After 90 days of hydrogen bathing, the depth of wrinkles is significantly reduced!
Hsiyu commented that although this study is very interesting, it is the first human experiment on skin aging after all. However, there are some flaws in the study, insufficient evidence from cytological tests, and no discussion on the reasons for collagen synthesis. In fact, the way in which skin cells synthesize collagen is relatively clear. How hydrogen affects these pathways is very easy to analyze and study, at least Detect some of the more important signal molecules.
The human test sample size is too small, only 6 and only 4 are effective. There must be 10-20 people in order to be considered as a small sample test. Such a small-scale test cannot prove the effectiveness of this method. It is difficult to rule out the interference of accidental and human factors.
